Distributed address auto configuration protocol for Manet networks

In heterogeneous network environments, the network connections of a multi-homed device may have significant bandwidth differential. For a multi-homed transmission protocol designed for network failure tolerance, such as SCTP, path selection algorithms for data transmission drastically affect performance. This article studies the effect of path bandwidth differential on the performance of retransmission strategies in multi-homing environments. It identifies that fast retransmission on an alternate path may cause receive buffer blocking when path bandwidth differential is significant and the receive buffer is limited. A theoretical model is proposed for selecting retransmission path during the fast retransmission phase, based on receive buffer and path conditions. From these observations and analysis results, this article proposes that path selection strategies for transmitting new data and retransmitted data should be decoupled. A new path selection scheme is proposed and evaluated through SCTP simulations.     

Hibiscus sabdariffa L: source of antioxidant dietary fiber

Dietary fiber and antioxidants are food constituents and functional ingredients that are generally addressed separately. Nowadays there is scientific evidence that primary characteristics of DF assigned to non starch polysaccharides and lignin (resistance to digestion and absorption in the small intestine and fermentation in the large intestine) can be extended to other indigestible food constituents that are resistant to digestion and absorption in the human small intestine with complete or partial fermentation in the large intestine. The antioxidant dietary fiber concept was defined as a dietary fiber concentrate containing significant amounts of natural antioxidants associated with non digestible compounds. Hibiscus sabdariffa L flower shows in its composition an important percentage of dietary fiber and high antioxidant capacity. The infusion obtained by decoction of flowers, had been extensibility studied due to the healthy properties. In this work the principal nutritional aspects from Hibiscus sabdariffa L and its use as a possible antioxidant dietary fiber source had been considered.     

Nanoimprint lithography fabrication of waveguide-integrated optical gratings with inexpensive stamps

We demonstrate that a replica grating can be effectively used as an inexpensive stamp for nanoimprint lithography to pattern diffractive optical couplers integrated with planar optical waveguides. Imprinted grating patterns were integrated with silicon oxynitride waveguide films to be used as an evanescent wave sensor in the input grating-coupler configuration. An anti-adhesion layer using an inexpensive, two-step chemical functionalization was developed for the stamps. The stamps were able to withstand imprint temperatures ranging from 140 to 190 °C and high fidelity imprints were obtained. The groove pattern was integrated in waveguide films by etch transfer and light-coupling properties of gratings with 1.2 μm pitch were tested using a λ = 1.55 μm laser. Compared to etched silicon masters, replica optical gratings provide uniform pattern density over their entire surface with no unstructured regions, are inexpensive, and readily available for R&D use.     

Comparison of biogenic amine profile in cheeses manufactured from fresh and stored (4 degrees C, 48 hours) raw goat's milk

In this study, the evolution of microbial counts, biogenic amine contents, and related parameters (pH, moisture, and proteolysis) in goat cheese made from fresh raw milk or raw milk stored for 48 h at 4 degrees C was examined. In both cases the milk was nonpasteurized. This study was designed to evaluate the effect of milk quality on the profile of biogenic amines in relation to the evolution of the microbial population during cheese making. Cheese made from raw milk stored for 48 h at 4 degrees C showed the highest microbial counts and biogenic amine levels. The storage of milk under refrigeration caused significant increases in the levels of some microbial and biogenic amines during ripening, but not initially. Tyramine was the main biogenic amine in the two cheeses tested, followed by cadaverine. However, the main differences in amine contents between batches were found for putrescine, histamine, and beta-phenylethylamine, whose levels were more than twofold higher in samples from raw milk refrigerated for 48 h than in samples from fresh milk.     

Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae

To identify new genes involved in acetate uptake in Saccharomyces cerevisiae, an analysis of the gene expression profiles of cells shifted from glucose to acetic acid was performed. The gene expression reprogramming of yeast adapting to a poor non-fermentable carbon source was observed, including dramatic metabolic changes, global activation of translation machinery, mitochondria biogenesis and the induction of known or putative transporters. Among them, the gene ADY2/YCR010c was identified as a new key element for acetate transport, being homologous to the Yarrowia lipolytica GPR1 gene, which has a role in acetic acid sensitivity. Disruption of ADY2 in S. cerevisiae abolished the active transport of acetate. Microarray analyses of ady2Delta strains showed that this gene is not a critical regulator of acetate response and that its role is directly connected to acetate transport. Ady2p is predicted to be a membrane protein and is a valuable acetate transporter candidate.     

Morphological and palynological assessment of taxonomically problematic genus Paspalum based on light and scanning electron microscopy

Present research work was carried out to clarify the variations among species of genus Paspalum morphologically and palynologically as this genus is taxonomically difficult due to having multiple similar morphologically overlapping characters which make it difficult to identify. Henceforth present research work was carried out to delimit taxa within the same genus by morphological and palynological tools through light microscopy (LM) and scanning electron microscopy (SEM). Both these tools are considered as the most useful taxonomic characters for taxonomically problematic genera. The results showed a lot of variations among morphological characters. In Paspalum dilatatum, the upper glume was ovate whereas in the other two species, the upper glume was elliptic. The upper glume apex found in P. dilatatum and Paspalum scrobiculatum was obtuse whereas in Paspalum distichum, upper glume apex was acute. Glume nerves showed variation in all three species. Paspalum distichum was 3 nerved, P. scrobiculatum was 5–7 nerved, and P. dilatatum was 5–9 nerved. All three species showed variation in lemma nerves. Paspalum scrobiculatum had 3 nerved lemma whereas in P. distichum 3–5 nerved and P. dilatatum 5–9 nerved lemma were present. In polar and equatorial view, pollen grains ranged from 25 (20–30) to 37.5 (30–45) μm. Paspalum distichum appeared to be the smallest in size whereas P. dilatatum was the largest. Exine thickness ranged from 0.75 (0.5–1) to 1.35 (1.2–1.5) μm. The higher value of pollen fertility was found in P. scrobiculatum as 87.69% and the lowest value was in P. distichum as 78.08%. Morphological keys were also given for correct identification.     

Fabrication,characterization and evaluation of the effect of PLGA and PLGA–PEG biomaterials on the proliferation and neurogenesis potential of human neural SH-SY5Y cells

In recent years with regard to the development of nanotechnology and neural stem cell discovery, the combinatorial therapeutic strategies of neural progenitor cells and appropriate biomaterials have raised the hope for brain regeneration following neurological disorders. This study aimed to explore the proliferation and neurogenic effect of PLGA and PLGA–PEG nanofibers on human SH-SY5Y cells in in vitro condition. Nanofibers of PLGA and PLGA–PEG biomaterials were synthesized and fabricated using electrospinning method. Physicochemical features were examined using HNMR, FT-IR, and water contact angle assays. Ultrastructural morphology, the orientation of nanofibers, cell distribution and attachment were visualized by SEM imaging. Cell survival and proliferation rate were measured. Differentiation capacity was monitored by immunofluorescence staining of Map-2. HNMR, FT-IR assays confirmed the integration of PEG to PLGA backbone. Water contact angel assay showed increasing surface hydrophilicity in PLGA–PEG biomaterial compared to the PLGA substrate. SEM analysis revealed the reduction of PLGA–PEG nanofibers' diameter compared to the PLGA group. Cell attachment was observed in both groups while PLGA–PEG had a superior effect in the promotion of survival rate compared to other groups (p < .05). Compared to the PLGA group, PLGA–PEG increased the number of Ki67⁺ cells (p < .01). PLGA–PEG biomaterial induced neural maturation by increasing protein Map-2 compared to the PLGA scaffold in a three-dimensional culture system. According to our data, structural modification of PLGA with PEG could enhance orientated differentiation and the dynamic growth of neural cells.     

Impact of Particle Generation Method on the Apparent Hygroscopicity of Insoluble Mineral Particles

Calcite (CaCO ₃ ) mineral particles are commonly generated by atomization techniques to study their heterogeneous chemistry, hygroscopicity, and cloud nucleation properties. Here we investigate the significant artifact introduced in generating calcium mineral particles through the atomization of a saturated suspension of the powder in water, by measuring particle hygroscopicity via CCN activation curves. Particles produced from atomization displayed hygroscopicities as large as κ_app > 0.1, 100 times more hygroscopic than that obtained for dry-generated calcite, κ_app = 0.0011. The hygroscopicity of the wet-generated particles increased as a function of time the calcite powder spent in water, and with decreasing particle size. Wet-generated calcium oxalate was more hygroscopic through wet- (κ_app = 0.34) versus dry-generation (κ_app = 0.048). Atomized calcium sulfate particles, however, were only slightly more hygroscopic (κ_app = 0.0045) than those generated dry (κ_app = 0.0016). Single-particle analysis by ATOFMS and SEM/EDX, and bulk analysis of the calcite powders by ICP-MS and IC revealed no significant soluble contaminants. The atomized particles were likely composed of components that dissolved from the powder and then re-precipitated, and appeared to contain little of the original mineral powder. The increased hygroscopicity of atomized calcite may have been caused by aqueous carbonate chemistry producing Ca(OH) ₂ , Ca(HCO ₃ ) ₂ , and metastable hydrates with increased solubility. Surface water adsorption may have also played a role, in addition to uncharacterized soluble components produced by wet-generation, and the precipitation of amorphous phases including glassy states. This study suggests that using wet-generation methods to suspend mineral dust samples will not produce particles with the correct physicochemical properties in laboratory studies, a finding which has important implications for past and future laboratory studies focusing on understanding relationships between the hygroscopicity and chemistry of mineral dust particles.     

Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems Optimization

Photo-polymerized hydrogels are ideally suited for stem-cell based tissue regeneration and three dimensional (3D) bioprinting because they can be highly biocompatible, injectable, easy to use, and their mechanical and physical properties can be controlled. However, photo-polymerization involves the use of potentially toxic photo-initiators, exposure to ultraviolet light radiation, formation of free radicals that trigger the cross-linking reaction, and other events whose effects on cells are not yet fully understood. The purpose of this study was to examine the effects of hydrogen sulfide (H₂S) in mitigating cellular toxicity of photo-polymerization caused to resident cells during the process of hydrogel formation. H₂S, which is the latest discovered member of the gasotransmitter family of gaseous signalling molecules, has a number of established beneficial properties, including cell protection from oxidative damage both directly (by acting as a scavenger molecule) and indirectly (by inducing the expression of anti-oxidant proteins in the cell). Cells were exposed to slow release H₂S treatment using pre-conditioning with glutathione-conjugated-garlic extract in order to mitigate toxicity during the photo-polymerization process of hydrogel formation. The protective effects of the H₂S treatment were evaluated in both an enzymatic model and a 3D cell culture system using cell viability as a quantitative indicator. The protective effect of H₂S treatment of cells is a promising approach to enhance cell survival in tissue engineering applications requiring photo-polymerized hydrogel scaffolds.